3,3-diaminobenzidine tetrahydrochloride

86,16

 

≥98 %, p.a.
Substrate of horseradish peroxidase. For detection of endogenous peroxidase and for immunohistochemical stainings.
3,3',4,4'-Tetra-aminobiphenyl tetrahydrochloride, DAB

 

  • C12H14N4 · 4 HCl
  • M 360,10 g/mol
  • mp >300 °C
  • store at +4 °C
  • WGK 1
  • EG-No. 231-018-9
  • CAS-No. [7411-49-6]
  • Warning H315-H319-H341-H351-H335

Popular substrate of peroxidases. DAB is used for biochemical and histological detection of endogenous peroxidases like catalase and cytochome oxidase. Additionally, it is the most common substrate for horseradish peroxidase (HRP) in histochemical and cytological assays. DAB is oxidised by HRP, forming a brown precipitate, which is unsoluble in aqueous and organic solvents. This precipitate can be detected in visible light and does not bleach during long-term storage. Application: Stock solution: 1-2 % (10-20 mg/ml) in 50 mM Tris buffer, pH 7.3 Storage of stock solution in aliquots at -20 °C Working concentration: 0.05-0.1 % (0.5-1 mg/ml) DAB in buffer (PBS, TBS, pH 7.0-7.6) containing 0.01 % hydrogen peroxide Always use freshly prepared working solution. For oxidation high concentrations of fresh hydrogen peroxide are needed.